منابع مشابه
Nudix hydrolases degrade protein-conjugated ADP-ribose
ADP-ribosylation refers to the transfer of the ADP-ribose group from NAD(+) to target proteins post-translationally, either attached singly as mono(ADP-ribose) (MAR) or in polymeric chains as poly(ADP-ribose) (PAR). Though ADP-ribosylation is therapeutically important, investigation of this protein modification has been limited by a lack of proteomic tools for site identification. Recent work h...
متن کاملMolecular characterization of organelle-type Nudix hydrolases in Arabidopsis thaliana
Nudix (nucleoside diphosphates linked to some moiety X) hydrolases act to hydrolyze ribo-and deoxyribo-nucleoside triphophates, nucleotide sugars, coenzymes, or dinucleoside polyphosphates. Arabidopsis thaliana contains 27 genes encoding Nudix hydrolase homologues (AtNUDX1-27) with a predicted distribution in the cytosol, mitochondria, and chloroplasts. Previously, cytosolic Nudix hydrolases He...
متن کاملA continuous fluorescence assay for the characterization of Nudix hydrolases
The common substrate structure for the functionally diverse Nudix protein superfamily is nucleotide-diphosphate-X, where X is a large variety of leaving groups. The substrate specificity is known for less than 1% of the 29,400 known members. Most activities result in the release of an inorganic phosphate ion or of a product bearing a terminal phosphate moiety. Reactions have typically been moni...
متن کاملSubstrate specificity characterization for eight putative nudix hydrolases. Evaluation of criteria for substrate identification within the Nudix family
The nearly 50,000 known Nudix proteins have a diverse array of functions, of which the most extensively studied is the catalyzed hydrolysis of aberrant nucleotide triphosphates. The functions of 171 Nudix proteins have been characterized to some degree, although physiological relevance of the assayed activities has not always been conclusively demonstrated. We investigated substrate specificity...
متن کاملThe 26 Nudix hydrolases of Bacillus cereus, a close relative of Bacillus anthracis.
The genome of Bacillus cereus contains 26 Nudix hydrolase genes, second only to its closest relative, Bacillus anthracis which has 30. All 26 genes have been cloned, 25 have been expressed, and 21 produced soluble proteins suitable for analysis. Substrates for 16 of the enzymes were identified; these included ADP-ribose, diadenosine polyphosphates, sugar nucleotides, and deoxynucleoside triphos...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2021
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.2021.35.s1.04546